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Vag8, a Bordetella pertussis bvg -Regulated Protein
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ABSTRACT
Bordetella pertussis
expresses a
bvg
-regulated 95-kDa protein, Vag8, encoded by
vag-8
. Southern blot analysis indicates that strains of
Bordetella bronchiseptica
and
Bordetella parapertussis
have DNA homologous to
vag-8
. Antiserum raised to a fusion of maltose binding protein to an N-terminal 60-kDa fragment of Vag8 recognizes the native 95-kDa protein in immunoblots of
B. pertussis
and
B. bronchiseptica
but not
B. parapertussis
. A 95-kDa protein-negative derivative of
B. pertussis
18323 containing a deletion of
vag-8
colonized mice as efficiently as the parent
B. pertussis
strain in a mouse aerosol model of pertussis.
Title: Vag8, a
Bordetella pertussis bvg
-Regulated Protein
Description:
ABSTRACT
Bordetella pertussis
expresses a
bvg
-regulated 95-kDa protein, Vag8, encoded by
vag-8
.
Southern blot analysis indicates that strains of
Bordetella bronchiseptica
and
Bordetella parapertussis
have DNA homologous to
vag-8
.
Antiserum raised to a fusion of maltose binding protein to an N-terminal 60-kDa fragment of Vag8 recognizes the native 95-kDa protein in immunoblots of
B.
pertussis
and
B.
bronchiseptica
but not
B.
parapertussis
.
A 95-kDa protein-negative derivative of
B.
pertussis
18323 containing a deletion of
vag-8
colonized mice as efficiently as the parent
B.
pertussis
strain in a mouse aerosol model of pertussis.
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