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Characterization of chromosomes and localization of the rDNA locus in the aye-aye (Daubentonia madagascariensis)
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The karyotype of a prosimian primate, the aye-aye (Daubentonia madagascariensis) is described. Results from a variety of staining methods (Q-, R-, G-, and C-banding, distamycin A/DAPI and methyl-green/DAPI) are reported. Sites of methyla-tion were visualized using antibodies against 5-methylcytosine. Digestion of aye-aye fixed metaphase chromosomes with the restriction endonuclease Haelll produced G-banding. No other restriction enzymes tested produced clear G- or C-banding patterns. Ag-staining of the nucleolar organizer regions (NORs) revealed the location of rDNA sites on the short arms of the smallest pairs of acrocentric chromosomes, 13p and 14p.
Title: Characterization of chromosomes and localization of the rDNA locus in the aye-aye (Daubentonia madagascariensis)
Description:
The karyotype of a prosimian primate, the aye-aye (Daubentonia madagascariensis) is described.
Results from a variety of staining methods (Q-, R-, G-, and C-banding, distamycin A/DAPI and methyl-green/DAPI) are reported.
Sites of methyla-tion were visualized using antibodies against 5-methylcytosine.
Digestion of aye-aye fixed metaphase chromosomes with the restriction endonuclease Haelll produced G-banding.
No other restriction enzymes tested produced clear G- or C-banding patterns.
Ag-staining of the nucleolar organizer regions (NORs) revealed the location of rDNA sites on the short arms of the smallest pairs of acrocentric chromosomes, 13p and 14p.
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