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Attachment of Cryptosporidium parvum Sporozoites to Human Intestinal Epithelial Cells
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ABSTRACT
An enzyme-linked immunosorbent assay-based attachment model using the human intestinal cell line Caco-2A was developed to study attachment of
Cryptosporidium parvum
sporozoites in vitro and to assess potential inhibitors of sporozoite binding. In this system, attachment was related to sporozoite dose, incubation time, and host cell differentiation status. Polyclonal antibodies to
C. parvum
as well as glycoprotein inhibitors of a sporozoite lectin reduced attachment. This model will be a valuable tool in elucidating specific molecules and mechanisms involved in sporozoite-host cell attachment.
American Society for Microbiology
Title: Attachment of
Cryptosporidium parvum
Sporozoites to Human Intestinal Epithelial Cells
Description:
ABSTRACT
An enzyme-linked immunosorbent assay-based attachment model using the human intestinal cell line Caco-2A was developed to study attachment of
Cryptosporidium parvum
sporozoites in vitro and to assess potential inhibitors of sporozoite binding.
In this system, attachment was related to sporozoite dose, incubation time, and host cell differentiation status.
Polyclonal antibodies to
C.
parvum
as well as glycoprotein inhibitors of a sporozoite lectin reduced attachment.
This model will be a valuable tool in elucidating specific molecules and mechanisms involved in sporozoite-host cell attachment.
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