#2959 Correlation of urinary MCP-1 with different histopathological classes of lupus nephritis

Abstract Background and Aims Lupus nephritis (LN) affects almost two-thirds of Systemic lupus erythematosus (SLE) patients. Renal biopsy is the gold standard for the classification of LN. Minimally invasive techniques, as urinary biomarkers, are promising tools for the diagnosis and monitoring of SLE. This study aimed to determine urinary MCP-1 level association with different LN histopathological classes. Method This study included 40 patients with active lupus nephritis proteinuria >0.5 gm and/or active urinary sediment and/or decline in kidney function indicated for renal biopsy by the nephrology team. Morning urine samples were collected to measure MCP-1 level by enzyme-linked immunosorbent assay (ELISA). Full blood cell count, serum creatinine level, blood urea nitrogen (BUN) was collected. Biopsies were classified according to ISN /RPS, and biopsy results were correlated with MCP-1 level. We excluded patients with overlap syndrome, active infection, and malignancy. All the patients were diagnosed according to the American College of Rheumatology (ACR) diagnostic criteria. Results Mean age 28.75 ± 6.37 years. Female patients were 33 (82.5%) and 7 (17.5%) male patients. Mean creatinine was 2.71 ± 0.55 mg/dl in the active cases and 0.84 ± 0.10 mg/dl in the control group. The mean albumin was 3.1 ± 0.61 mg/dl in the active cases, and 4.05 ± 0.42 mg/dl in the control group. The mean eGFR was 56.14 ± 20.40 ml/min/1.73 m in the active cases, and 99.63 ± 6.90 40 ml/min/1.73 m in the control group. Our study showed a significant correlation between disease activity and urinary MCP-1 level. The Median MCP-1 level was 618.4 ng/l in patients with active LN and 120.05 ± 87.53 ng/l in patients with inactive LN with a sensitivity of 97.5% and specificity 95%. Level of urinary MCP-1 was highest in class IV with median of 998 followed by class III 567 then class V with median 357 ng/ml Conclusion Urinary MCP-1 level provided an auxiliary noninvasive tool to distinguish between different histopathological classes of active LN.