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Supplemental Figure 6 from DNA-Directed Polymerase Subunits Play a Vital Role in Human Telomeric Overhang Processing
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<p>Supplemental Figure 6. A. Western blot for HeLaII transfected with the siRNAs indicated on top. The analysis was performed 48 hours post-transfection. B. Telomeric overhang analysis in HelaII depleted for 180 with the indicated siRNAs, using the DSN (double strandspecific nuclease) technique. Briefly, four micrograms of genomic DNA were digested with 0.8 U of double strand-specific nuclease (DSN) as per manufacturer instructions, ran on a 6% PAGE, transferred to a membrane, and hybridized to a C-rich (CCCTAA) probe. Undigested and ExoI controls were ran alongside, as labeled below the gel. See materials and methods, and (32) for further details. C. Quantitation of the overhangs detected in panel B, with values from three independent experiments. The two-tailed Student t test for the two significant values (<0.05) are shown.</p>
American Association for Cancer Research (AACR)
Title: Supplemental Figure 6 from DNA-Directed Polymerase Subunits Play a Vital Role in Human Telomeric Overhang Processing
Description:
<p>Supplemental Figure 6.
A.
Western blot for HeLaII transfected with the siRNAs indicated on top.
The analysis was performed 48 hours post-transfection.
B.
Telomeric overhang analysis in HelaII depleted for 180 with the indicated siRNAs, using the DSN (double strandspecific nuclease) technique.
Briefly, four micrograms of genomic DNA were digested with 0.
8 U of double strand-specific nuclease (DSN) as per manufacturer instructions, ran on a 6% PAGE, transferred to a membrane, and hybridized to a C-rich (CCCTAA) probe.
Undigested and ExoI controls were ran alongside, as labeled below the gel.
See materials and methods, and (32) for further details.
C.
Quantitation of the overhangs detected in panel B, with values from three independent experiments.
The two-tailed Student t test for the two significant values (<0.
05) are shown.
</p>.
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