Phytochemical and Antimicrobial Activity Screening of StemExtracts of Tinospora cordifolia

Abstract-Tinosporacordifoliaisapopularmedicinal plant which is used in several traditional medicines to cure various diseases. The common names are Amrita and Guduchi, belonging to the family of Menispermaceae. The aim of the study was to study the stem extract of traditional medicinal plant, T. cordifolia for qualitative estimation of phytoconsituents and subsequently to determine its antibacterialactivityagainsttwotestmicroorganisms Escherichia coliand Pseudomonas aeruginosato authenticate its use in traditional medicines. Stems were air-dried and coarsely powdered samples were subjectedto Soxhlet extractionusingdiversesolvents(Hexane,chloroform, ethyl acetate and methanol and water). Freshly prepared extracts were exposed to standard phytochemical analysis for qualitative estimation of phytoconsituents. The antibacterial activity of the stem extract of T. cordifolia was determined by agar well diffusion method. Phytochemical analysis revealed the presence of several phytochemicals viz., alkaloids, flavonoids, steroids, phenol, tannins, steroid, terpenoids, saponins and sugars. The methanolic extract displayed the presence of highest number of phytochemical compounds. It may be due to the higher solubility of active components in this solvent as compared to other solvents. The results revealed that the methanolic extract exhibit the effective antibacterial activity against the tested bacterial species . The studies justify the use of T. cordifolia in traditional medicines. The investigation further proposed that the metabolites present in leaf tissue of T. cordifolia can be potential source of novel natural antibacterial and antioxidant agents and can be prospective applications in food industry as an antioxidant