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Modified Bacterial Conjugation Protocol For Pseudo-nitzschia multiseries v2
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This is a modified version of 'Conjugation of Thalassiosira pseudonana', publishedby J. Turnsekdx.doi.org/10.17504/protocols.io.f55bq86. The protocol was modified to enhance viability in the diatom Pseudo-nitzschia multiseries. This version was used to transform Pseudo-nitzschia multiseries isolate 15091C3 with an episomal plasmid derived from pPtPUC3 to express a egfp gene under control of the P. multiseries actin promoter and termination domains. PLASMID INFO pPmAGFPC10 EPI : A P. multiseries derived expression cassette under control of P. multiseries ca 1000 bp of actin promoter and termination domains. Pm actPs::eGFP::actTs
Title: Modified Bacterial Conjugation Protocol For Pseudo-nitzschia multiseries v2
Description:
This is a modified version of 'Conjugation of Thalassiosira pseudonana', publishedby J.
Turnsekdx.
doi.
org/10.
17504/protocols.
io.
f55bq86.
The protocol was modified to enhance viability in the diatom Pseudo-nitzschia multiseries.
This version was used to transform Pseudo-nitzschia multiseries isolate 15091C3 with an episomal plasmid derived from pPtPUC3 to express a egfp gene under control of the P.
multiseries actin promoter and termination domains.
PLASMID INFO pPmAGFPC10 EPI : A P.
multiseries derived expression cassette under control of P.
multiseries ca 1000 bp of actin promoter and termination domains.
Pm actPs::eGFP::actTs.
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