Javascript must be enabled to continue!
Fast Selective Detection of Pyocyanin Using Cyclic Voltammetry
View through CrossRef
Pyocyanin is a virulence factor uniquely produced by the pathogen Pseudomonas aeruginosa. The fast and selective detection of pyocyanin in clinical samples can reveal important information about the presence of this microorganism in patients. Electrochemical sensing of the redox-active pyocyanin is a route to directly quantify pyocyanin in real time and in situ in hospitals and clinics. The selective quantification of pyocyanin is, however, limited by other redox-active compounds existing in human fluids and by other metabolites produced by pathogenic bacteria. Here we present a direct selective method to detect pyocyanin in a complex electroactive environment using commercially available electrodes. It is shown that cyclic voltammetry measurements between −1.0 V to 1.0 V reveal a potential detection window of pyocyanin of 0.58–0.82 V that is unaffected by other redox-active interferents. The linear quantification of pyocyanin has an R2 value of 0.991 across the clinically relevant concentration range of 2–100 µM. The proposed method was tested on human saliva showing a standard deviation of 2.5% ± 1% (n = 5) from the known added pyocyanin concentration to the samples. This inexpensive procedure is suggested for clinical use in monitoring the presence and state of P. aeruginosa infection in patients.
Title: Fast Selective Detection of Pyocyanin Using Cyclic Voltammetry
Description:
Pyocyanin is a virulence factor uniquely produced by the pathogen Pseudomonas aeruginosa.
The fast and selective detection of pyocyanin in clinical samples can reveal important information about the presence of this microorganism in patients.
Electrochemical sensing of the redox-active pyocyanin is a route to directly quantify pyocyanin in real time and in situ in hospitals and clinics.
The selective quantification of pyocyanin is, however, limited by other redox-active compounds existing in human fluids and by other metabolites produced by pathogenic bacteria.
Here we present a direct selective method to detect pyocyanin in a complex electroactive environment using commercially available electrodes.
It is shown that cyclic voltammetry measurements between −1.
0 V to 1.
0 V reveal a potential detection window of pyocyanin of 0.
58–0.
82 V that is unaffected by other redox-active interferents.
The linear quantification of pyocyanin has an R2 value of 0.
991 across the clinically relevant concentration range of 2–100 µM.
The proposed method was tested on human saliva showing a standard deviation of 2.
5% ± 1% (n = 5) from the known added pyocyanin concentration to the samples.
This inexpensive procedure is suggested for clinical use in monitoring the presence and state of P.
aeruginosa infection in patients.
Related Results
Protein kinase activities in rat pancreatic islets of Langerhans
Protein kinase activities in rat pancreatic islets of Langerhans
1. Protein kinase activities in homogenates of rat islets of Langerhans were studied. 2. On incubation of homogenates with [gamma-32P]ATP, incorporation of 32P into protein occurre...
Pesticide Identification through PCA-ANN Classification of Cyclic Voltammetry using Screen-Printed Electrodes
Pesticide Identification through PCA-ANN Classification of Cyclic Voltammetry using Screen-Printed Electrodes
Introduction
Pollution caused by the use of pesticides is a growing concern, strongly linked to the expansion of agricultural operat...
Analysis Of Existing Cyclic Vertical Load Tests For Piles In Clay
Analysis Of Existing Cyclic Vertical Load Tests For Piles In Clay
ABSTRACT
A data base is collected to study the behavior of piles in clay under cyclic axial loads generated by ocean waves. The data base includes 16 studies on c...
Histamine‐induced increases in cyclic AMP levels in bovine adrenal medullary cells
Histamine‐induced increases in cyclic AMP levels in bovine adrenal medullary cells
The effect of histamine on cellular cyclic AMP levels in cultured bovine adrenal medullary cells has been studied.
Histamine (0.3–30 μm) increased cyclic AMP levels transiently, wi...
Structure of BrlR reveals a potential pyocyanin binding site
Structure of BrlR reveals a potential pyocyanin binding site
AbstractThe transcriptional regulator BrlR from Pseudomonas aeruginosa is a member of the MerR family of multidrug transport activators. Studies have shown BrlR plays an important ...
Overview: Electrochemical Sensors for Dopamine
Overview: Electrochemical Sensors for Dopamine
Dopamine (DA) is a neurotransmitter located in the ventral tegmental area of the midbrain, the substantia nigra pars compacta, and the arcuate nucleus of the hypothalamus of the hu...
Modifying platinum microelectrodes with electrochemically reduced graphene oxide for application in electrochemical ascorbic acid sensor
Modifying platinum microelectrodes with electrochemically reduced graphene oxide for application in electrochemical ascorbic acid sensor
Graphene oxide drop-casted on a platinum microelectrode was successfully changed to reduced graphene oxide by using a cyclic voltammetry method. The presence of an electrochemicall...
Two Possibly Distinct Prostaglandin E1 Receptors in N1E‐115 Clone: One Mediating Inositol Trisphosphate Formation, Cyclic GMP Formation, and Intracellular Calcium Mobilization and the Other Mediating Cyclic AMP Formation
Two Possibly Distinct Prostaglandin E1 Receptors in N1E‐115 Clone: One Mediating Inositol Trisphosphate Formation, Cyclic GMP Formation, and Intracellular Calcium Mobilization and the Other Mediating Cyclic AMP Formation
AbstractProstaglandin E1 (PGE1)‐mediated transmembrane signal control systems were investigated in intact murine neuroblastoma cells (clone N1E‐115). PGE1 increased intra‐cellular ...

