Pengaruh Puasa Intermiten Kering terhadap Gambaran Mikrostruktur Pankreas Mencit

Abstract. A lifestyle with minimal physical activity and consumption of high-fat foods canincrease the risk of obesity and insulin resistance, and can lead to high blood sugar levels anddestroy pancreatic beta cells. Intermittent fasting can significantly reduce insulin resistanceand maintain pancreatic beta cell mass and function. This study aims to analyze the effect ofdry intermittent fasting on the microstructure of the pancreas of mice (Mus Musculus L.) fedhigh-fat feed. This study is an experimental research with a post-test only controlled groupdesign approach, using a research sample of stored biological material (BBT) in the form of24 paraffin blocks of pancreatic tissue. The study sample group was divided into four: thecontrol sample was fed standard feed without dry intermittent fasting, the control sample wasfed high-fat without dry intermittent fasting, the sample was given standard feed with dryintermittent fasting, and the sample was given high-fat feed with dry intermittent fasting. The results of the study were statistically analyzed by the Kruskal-Walis nonparametic test. Fromthe results of the study, it can be concluded that dry intermittent fasting is thought to decreasepancreatic compensation which is reflected in the reduced number  of islets of langerhanscompared to the group that was fed high-fat feed without dry intermittent fasting. Abstrak. Pola hidup dengan aktivitas fisik minim dan konsumsi makanan tinggi lemak dapat  meningkatkan resiko terjadinya obesitas dan resistensi insulin, dan dapat menyebabkan kadar gula darah tinggi serta menghancurkan sel beta pankreas. Puasa Intermiten secara signifikan dapat mengurangi resistensi insulin dan mempertahankan massa dan fungsi sel beta pankreas. Penelitian ini bertujuan untuk menganalisis pengaruh puasa intermiten kering terhadap gambaran mikrostruktur pankreas mencit (Mus Musculus L.) yang diberi pakan tinggi lemak. Penelitian ini merupakan penelitian eksperimental dengan pendekatan  post-test only controlled group design, menggunakan sampel penelitian adalah bahan biologi tersimpan (BBT) berupa 24 parafin blok jaringan pankreas. Kelompok sampel penelitian dibagi menjadi empat: sampel kontrol diberi pakan standar tanpa puasa intermiten kering, sampel kontrol diberi pakan tinggi lemak tanpa puasa intermiten kering, sampel diberi pakan standar dengan puasa intermiten kering , dan sampel diberi pakan tinggi lemak dengan puasa intermitenkering. Hasil penelitian dianalisis secara statistik oleh uji nonparametik Kruskal-Walis. Dari hasil penelitian, dapat disimpulkan, puasa intermiten kering diduga menurunkan kompensasi pankreas yang tercemin dari berkurangnya jumlah islet of langerhans dibandingkan dengan kelompok yang diberi pakan tinggi lemak tanpa berpuasa intermiten kering.